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. Author manuscript; available in PMC: 2008 Jun 8.
Published in final edited form as: J Mol Biol. 2007 Jan 27;369(3):696–709. doi: 10.1016/j.jmb.2007.01.060

Figure 1.

Figure 1

Alanine-substitution scanning of the B2.1 sequence. (a) Binding of IgG b12 (grey bars) and Fab b12 (black bars) to phage displaying Ala-substituted B2.1 sequences. (b) Binding of sera from each of three mice (grey, black, and white bars) immunized with B2.1 synthetic peptide-OVA conjugate. Results are expressed as % binding of each mutant phage with respect to wild-type (wt) B2.1 phage; f88 is a negative control for phage bearing only wild-type pVIII protein. *Ala replacement of the Cys residue was not included in this study, as previous work showed substitution of this Cys by Ser abrogates b12 binding.21