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. Author manuscript; available in PMC: 2008 Jul 1.
Published in final edited form as: Cell Signal. 2007 Feb 8;19(7):1542–1553. doi: 10.1016/j.cellsig.2007.01.028

Fig. 1. IGF-I upregulates COX-2 mRNA expression.

Fig. 1

(A) A2780 and OVCAR-3 cells were cultured in serum-free medium for 16 h, then treated with the indicated concentrations of IGF-I for 6 h. COX-2 and GAPDH mRNA levels in the cells were detected by RT-PCR. (B) The cells were treated with 200 ng/ml of IGF-I for 0 to 24 h as indicated. COX-2 and GAPDH mRNA expression was analyzed by RT-PCR. The levels of COX-2 mRNA were quantified using NIH Image J software, and normalized to those of GAPDH levels. The mean ± SD of the corresponding densitometry data from replicate experiments were showed in lower panels of each figure.