Skip to main content
. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Virology. 2007 Jan 26;362(1):109–119. doi: 10.1016/j.virol.2006.12.026

Figure 1. Effect of Hsp90 inhibition of VSV replication.

Figure 1

A) viral titers at 12hpi from cells infected with VSV at an MOI=0.01 and treated with increasing concentrations of geldanamycin. B) growth curve of VSV in the presence (△) or absence (■) of geldanamycin (5μM) at high MOI (=10) and C) growth curve at low MOI (=0.1). D) growth curve of VSV in the presence (△) or absence (■) of radicicol (5μM). Virus titers for all panels represent the average of three different experiments. E) Western blot analysis of 25ug of lysate from cells that were mock-transfected, transfected with GAPDH targeting siRNA or transfected with Hsp90 targeting siRNA. Top image shows Hsp90 levels, middle panel shows GAPDH levels, bottom panel shows actin as a loading control. F) growth curve of mock-transfected and siRNA-treated cells infected with VSV at an MOI of 0.01. Error bars reflect standard deviation for three experiments.