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. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Virology. 2007 Jan 26;362(1):109–119. doi: 10.1016/j.virol.2006.12.026

Figure 3. Assembly of VSV in geldanamycin treated cells.

Figure 3

Cells were infected with VSV for 5 hours, and then proteins were radiolabeled and followed using a one hour pulse and one hour chase protocol. Upon addition of radioactive methionine, different concentrations of geldanamycin were added to individual samples. Following a 1 hour chase in the presence of drug A) intracellular radiolabeling of protein was determined by SDS-PAGE electrophoresis phosphorescence imaging. B) The amount of newly synthesized protein that was incorporated into the virus was determined by virion purification from the media followed by SDS-PAGE and phosphorescence analysis.