Figure 4. Effect of geldanamycin on viral protein stability.
A) BHK cells infected with VSV for 5 hours were starved for methionine, and then protein synthesis and stability were determined by pulse chase analysis (see materials and methods). Results were determined by SDS-PAGE and phosphorescence imaging. Viral proteins are identified to the right of the gel. Levels of protein remaining are quantities for viral L (B) and M (C) proteins presence (△) or absence (■) of 5μM geldanamycin. Values represent the average of three separate experiments +/- standard deviation