Figure 5. Geldanamycin destabilization of VSV L protein requires proteosome function.
A) BHK cells were infected with VSV, and 5hpi left untreated, treated with geldanamycin, or treated with both geldanamycin and proteosome inhibitor. Pulse-chase results for 10 minute and 30 minute chases are shown. B) Stability of L protein in the proteins presence (△) or absence (■) of 5μM geldanamycin and in the presence of geldanamycin and a proteosome inhibitor (◆, dotted line). Levels are expressed as a percentage of the signal that was present at the 10 minute chase. Values are the average of three separate experiments +/- standard deviation.