Figure 7. Hsp90 inhibition destabilizes newly synthesized L proteins from multiple paramyxoviruses.

A) Cells were mock infected (M lane) or infected at an MOI of 10with rSV5-GFP. At 13 h pi, cells were treated for 1 hr with or without 1μM Geldanamycin. Cells were radiolabeled for 20 min with ³⁵S-amino acids and incubated in nonradioactive media for the indicated times before lysis and analysis by immunoprecipitation with antibodies specific for NP, P or L. Geldanamycin was included at 1 μM during radiolabeling and chase periods. B) Stability of L proteins. Cells were mock infected or infected at an MOI of 10 with SV5, HPIV-2, SV41 or HPIV-3. 13hpi, cells were treated with 1μM Geldanamycin, radiolabeled for 20 minutes and lysed (Pulse) or chased using unlabeled methionine for 1 hour (Chase). Following cell lysis, samples were immunoprecipitated using L-specific antibodies. C) Experiments similar to B were carried out, but 1μM radicicol was used instead of geldanamycin