Figure 4. Dose-dependent inhibition of LPA-SA by EDTA and phenanthrolin.
Conditioned medium was collected and frozen after 24 h incubation with 3T3F442A adipocytes. LPA was quantified after 6 h additional incubation at 37°C of defrosted conditioned medium in the presence of increasing concentrations of EDTA (A) or phenanthrolin (B). Data represent the mean ± SE of 3 separate experiments. ** corresponds to P<0.01 (Student’s t test) when comparing to control values without EDTA (A) or without phenanthrolin (B).