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. Author manuscript; available in PMC: 2007 Nov 14.
Published in final edited form as: J Lipid Res. 2002 Jun;43(6):904–910.

Figure 5. Reversion of EDTA effect on LPA-SA by CaCl2 and CoCl2.

Figure 5

Conditioned medium was collected and frozen after 24 h incubation with 3T3F442A adipocytes. LPA was quantified either immediatly after defrosting (Initial) or 6h after (after 6h) furtherincubation at 37°C in the absence (cont, EDTA) or in the presence of 0.2 mM EDTA ± 5 mM of calcium chloride (CaCl2), magnesium chloride (MgCl2), or cobalt chloride (CoCl2). Data represent the mean ± SE of 3 separate experiments. * corresponds to P<0.05 and ** corresponds to P<0.001 when comparing to cont EDTA (Student’s t test).