Figure 6. Presence of a lysophospholipase D activity in adipocyte conditioned medium.
Conditioned medium was collected and frozen after 24 h incubation with 3T3F442A adipocytes. LPA was quantified either immediately after defrosting (Initial) or 6h after (after 6h) further incubation at 37°C in the absence (cont) or the presence of 2 μM oleoyl-lysophosphatidylcholine (LPC), or 2 μM oleoyllysophosphatidylglycerol (LPG), or 2 μM palmitoyl-lyso-platelet activating factor (LPAF), or 2 μM dioleoyl-phosphatidic acid (PA). Data represent the mean ± SE of 3 separate experiments. * corresponds to P<0.05 and ** corresponds to P<0.001 when compared to cont (Student’s t test).