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. 2001 Apr 15;107(8):995–1006. doi: 10.1172/JCI11717

Figure 7.

Figure 7

Administration of anti–VLA-4 during ongoing R-EAE enhances infiltration of CD4+ T cells into the CNS and upregulates the expression of VCAM-1. Lower thoracic spinal cord tissue sections from representative animals of the treatment groups described in Figure 1c were examined for infiltration of inflammatory cells on day 33 after immunization. Nine treatments (initiated on day 14) of control Ab or anti–VLA-4 had been given by this time point. Both animals shown had a clinical score of 2. DAPI, in blue, stains all cells; CD4+ cells and VCAM-1 are marked in red. (a) Spinal cord CD4+ infiltrate in a control Ab–treated animal. (b) Spinal cord CD4+ infiltrate in an anti–VLA-4–treated animal. (c) Spinal cord VCAM-1 expression in a control Ab–treated animal. (d) Spinal cord VCAM-1 expression in an anti–VLA-4–treated animal. Tissue from a naive animal was tested simultaneously with all three Ab’s and revealed the presence of only DAPI+ (blue) cells. All sections are 6 μm thick. ×100.