Figure 3. Activation of NADPH oxidase by spaced application of 5-HT.
A, 5-HT increases phosphorylation of p47phox subunit of the NADPH oxidase. Upper panels, example of Western blot assay. IP, immunoprecipitation; IB, immunoblot. Lower panel, ratio of p-serine to p47phox, average data of the densitometric analysis (mean ± s.e.m.) from three individual experiments run in triplicate. B, effects of spaced applications of 5-HT on NADPH oxidase activity. Results are mean ± s.e.m.,n = 5 experiments each with 5-HT (filled columns) and with combined application of 5-HT and ketanserin (Ketan; open columns). Asterisks represent P < 0.001 compared to untreated controls. n.s., not significant (P > 0.05).