Table 1.
Duration (s) | Number of peaks | Period (s) | Percentage of oscillatory cells | |
---|---|---|---|---|
Ca2+ oscillation in intact cells | ||||
2 μm UTP | 234 ± 24 | 12 ± 2 | 19 ± 0.7 * | 93 (14/15) |
10 μm UTP | 181 ± 31 | 11 ± 2 | 17 ± 0.9 | 50 (6/12) |
2 μm ATP | 196 ± 33 | 11 ± 2 | 17 ± 0.7 * | 100 (5/5) |
10 μm ATP | 177 ± 29 | 12 ± 2 | 15 ± 0.4 | 57 (4/7) |
Current oscillation in perforated whole-cell configuration | ||||
2 μm UTP | 413 ± 30 | 17 ± 4 | 28 ± 7.5 | 67 (4/6) |
10 μm UTP | 470 ± 55 | 21 ± 3 | 24 ± 4.0 | 100 (4/4) |
Current oscillation in ruptured whole-cell configuration | ||||
2 μm UTP | 209 ± 35 | 9 ± 2 | 23 ± 1.0 * | 88 (14/16) |
10 μm UTP | 161 ± 24 | 9 ± 1 | 18 ± 1.3 | 42 (5/12) |
Duration and number of peaks were defined as total time lapsed and the total number of peaks between the first and the final peaks. Period was calculated by dividing the duration by the number of peaks in each experiment. Only cells showing oscillations were included in the analysis.
Asterisks (P < 0.05) indicate that parameters at 2 μm UTP or ATP in same condition are significantly different compared with the values at 10 μm. Data are means ± s.e.m.