Skip to main content
. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: Vaccine. 2006 Nov 7;25(8):1409–1415. doi: 10.1016/j.vaccine.2006.10.043

Figure 4. The pKd minigene vaccine induces specific CTL killing of FLK-1+ endothelial cells.

Figure 4

(A) Surface expression of FLK-1 by the endothelial cell line HEVc and by HEVc cells stably transfected with FLK-1 (HEVc-FLK). Cells were washed and incubated with PE-conjugated isotype control Ab (thin grey lines), or PE-conjugated anti-FLK-1 Ab (thick black lines). Groups of immunized BALB/c mice (n=4) were sacrificed 2 wk after the last immunization and splenocytes isolated from them were stimulated with irradiated HEVc-FLK cells for 5 d. Thereafter, cytotoxicity assays were performed with parental HEVc (◇) or HEVc-FLK (■) as target cells. (B) Effector cells isolated from control group of mice immunized only with empty vector. (C) Effector cells from the pKd vaccinated group of mice. (D) Effector cells from the positive control group immunized with the FLK-1 whole gene vaccine.