Figure 5. The pKd minigene suppresses angiogenesis in syngeneic BALB/c mice.
(A) Masson’s trichrome staining of CT-26 lung metastasis tumor sections prepared 19 d after tumor cell challenge. The blue arrows indicate the blood vessels in the tumor. (B) Matrigel assay. Matrigel was implanted into mice vaccinated with either empty vector or pKd vaccines. Quantification of vessel growth by fluorimetry after staining of endothelium with FITC-labeled isolectin B4. The average fluorescence of Matrigel plugs from each group of mice is depicted by the bar graph (n=4; mean + SD). *, P<0.05 compared to empty vector control group.