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. Author manuscript; available in PMC: 2007 Oct 1.
Published in final edited form as: J Mol Biol. 2007 Mar 12;369(1):69–78. doi: 10.1016/j.jmb.2007.03.002

Figure 2. Sialylation of terminal GalNAc in the hinge region of IgA1 (Mce) by DAKIKI cell extracts.

Figure 2

Desialylated IgA1 (Mce) (DS:IgA) was incubated with a Golgi-enriched enzyme preparation from DAKIKI cells (DAKIKI+DS:IgA) and CMP-NeuAc and subsequently desialylated (DS:DAKIKI+DS:IgA). The samples, including DAKIKI Golgi preparations without added IgA1, were separated on SDS-PAGE, western-blotted onto a PVDF membrane and incubated with HAA lectin, that recognizes terminal GalNAc (A). To confirm that equivalent levels of IgA were loaded, the membrane was stripped and re-probed with an IgA-specific antibody (B).