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Esterase assisted probe activation. (a) Hydrolysis of probe 2 (1.9 μM) with and without esterase (10 units) at 25 °C. (b) Observed absorption changes at 500 nm after treating probe 2 with different amounts of esterase for different time periods (0, 5, 15, 30 min) at 25 °C. (b) Fluorescence intensity changes at 597 nm after treating probe 2 with increasing amounts of esterase (0, 1, 2, 4, 6, 8, 10 units) after 30 min at 25 °C.17
