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. 2006 Jun 29;575(Pt 3):707–720. doi: 10.1113/jphysiol.2006.114439

Figure 6. The NMDAR1 subunit of the NMDA glutaminergic receptor is present in ARPE-19 cells.

Figure 6

A, a monoclonal antibody against the extracellular loop of NMDAR1 (NR1; MAB363) produced clear and abundant staining (green). DAPI localized to cell nuclei (blue). Scale bar represents 20 μm. B, no staining was observed in the absence of the primary antibody (MAB363), while blue DAPI staining testified to the presence of cells. Scale bar represents 20 μm. C, higher magnification image shows diffuse staining for NMDAR1 with antibody MAB363 across the cell (green). Punctate green staining was also detected and was frequently around the nucleus, stained blue with DAPI. Scale bar represents 10 μm. D, similar staining was observed using a polyclonal antibody raised against the C-terminal of the NR1 subunit (AB1516; green). DAPI localizes cell nuclei (blue). Scale bar represents 20 μm. E, no staining was observed when the primary antibody, AB1516, was pre-absorbed with its specific peptide (AG344). Scale bar represents 20 μm.