Table 2.
Mass spectrometry and peptide-fingerprinting data for identified proteins that show significant changes after sham surgery or UL
| Spot | Protein | Mr | pl | MOWSE score | Hit/submitted | Intensity (%) | Mean ±s.d. (delta p.p.m.) |
|---|---|---|---|---|---|---|---|
| Group 1: affected by UL, but not by sham-op | |||||||
| 511 | Succinate dehydrogenase | 71.6 | 6.8 | 1.9E + 07 | 13/46 | 32 | 2 ± 3 |
| 513 | Succinate dehydrogenase | 71.6 | 6.8 | 2.4E + 02 | 4/27 | 13 | 6 ± 9 |
| 1222 | Prohibitin | 29.8 | 5.6 | 5.12E + 04 | 7/36 | 33 | 1 ± 1 |
| 1267 | Prohibitin | 29.8 | 5.6 | 1.50E + 06 | 8/39 | 33 | 7 ± 8 |
| 672 | Neuropilin-2 | 103.9 | 5.1 | 9.51E + 02 | 4/35 | 16 | 8 ± 4 |
| 94 | Dihydropyrimidase related protein-2 | 62.3 | 5.9 | 8.9E + 07 | 13/33 | 57 | 6 ± 7 |
| 14 | Dihydropyrimidase related protein-2/3 | 61.9 | 6.0 | 1.31E + 02 | 5/29 | 28 | 8 ± 10 |
| 655 | d-3-Phosphoglycerate dehydrogenase | 56.5 | 6.3 | 8.70E + 03 | 6/18 | 45 | 6 ± 8 |
| 238 | Triosephosphate isomerase | 26.9 | 6.4 | 5.70E + 04 | 8/12 | 82 | 6 ± 8 |
| 180 | Thioredoxin-like protein 2 | 31.4 | 4.9 | 2.82E + 02 | 4/16 | 37 | 9 ± 11 |
| 378 | Glutathione S-transferase P | 23.4 | 6.9 | 6.80E + 03 | 5/16 | 35 | 4 ± 6 |
| 508 | Phosphoglycerate mutase 1 | 28.6 | 6.2 | 1.1E + 04 | 6/22 | 38 | 6 ± 7 |
| 470 | Phosphoglycerate mutase 1 | 28.6 | 6.2 | 1.10E + 04 | 6/22 | 47 | 8 ± 10 |
| 468 | Phosphoglycerate mutase 1 | 28.6 | 6.2 | 4.16E + 02 | 4/55 | 22 | 3 ± 5 |
| Group 2: affected by UL, and by sham-op | |||||||
| 1079 | Voltage-dependent anion-selective channel protein 1 | 30.8 | 8.6 | 8.04E + 05 | 7/51 | 27 | 6 ± 7 |
| 1120 | Voltage-dependent anion- selective channel protein 1 | 30.8 | 8.6 | 5.60E + 08 | 12/40 | 49 | 4 ± 6 |
| 386 | Peroxiredoxin 2 | 21.8 | 5.3 | 1.00E + 04 | 6/18 | 61 | 7 ± 9 |
| 252 | Peroxiredoxin 2 | 21.8 | 5.3 | 1.2E + 06 | 9/20 | 60 | 6 ± 8 |
| 667 | Protein disulphide isomerase A3 | 56.6 | 5.9 | 2.92E + 09 | 15/45 | 36 | 4 ± 5 |
| 971 | α-Enolase | 47.2 | 5.8 | 1.11E + 03 | 6/12 | 79 | 5 ± 7 |
| 40 | Peroxiredoxin 6 | 24.8 | 5.6 | 8.3E + 04 | 9/26 | 54 | 6 ± 8 |
| 210 | Peroxiredoxin 6 | 24.8 | 5.6 | 2.2E + 05 | 8/21 | 56 | 3 ± 4 |
| 725 | Aldose reductase | 35.8 | 6.3 | 2.0E + 03 | 6/19 | 35 | 5 ± 6 |
| 329 | Creatine kinase | 47.0 | 8.7 | 3.1E + 05 | 10/19 | 56 | 3 ± 4 |
| 977 | Creatine kinase | 47.0 | 8.7 | 1.81E + 04 | 8/30 | 46 | 6 ± 8 |
| 322 | Creatine kinase | 47.0 | 8.7 | 2.20E + 04 | 8/28 | 60 | 6 ± 7 |
| 30 | Nucleolar transcription factor 1 | 89.4 | 5.6 | 1.4E + 01 | 5/61 | 7 | 6 ± 7 |
| 936 | Pantothenate kinase 4 | 86.2 | 5.9 | 5.24E + 01 | 4/16 | 12 | 6 ± 8 |
| 697 | Phosphoserine transeferase | 40.5 | 8.1 | 2.6E + 02 | 8/28 | 32 | 4 ± 5 |
| 231 | Carbonic anhydrase II | 29.1 | 6.9 | 5.48E + 04 | 7/48 | 20 | 7 ± 8 |
| 487 | Aconitate hydratase | 85.3 | 8.1 | 2.0E + 09 | 7/21 | 59 | 2 ± 3 |
| Group 3: affected by sham-op, but not by UL | |||||||
| 704 | Pyruvate kinase, isozymes M1/M2 | 57.8 | 6.6 | 2.25E + 04 | 9/27 | 32 | 6 ± 7 |
| 831 | Pyruvate kinase, isozymes M1/M2 | 57.8 | 6.6 | 5.70E + 05 | 10/53 | 21 | 9 ± 10 |
| 1085 | Creatine kinase, b-chain | 42.7 | 5.3 | 1.13E + 06 | 11/19 | 86 | 3 ± 4 |
| 842 | Adenylate kinase isozyme 1 | 21.6 | 7.7 | 2.5E + 02 | 5/75 | 13 | 5 ± 6 |
| 444 | Stress-induced phosphoprotein 1 | 62.6 | 6.4 | 4.7E + 04 | 13/58 | 40 | 4 ± 4 |
| 696 | Vacuolar ATP synthase subunit B, brain isoform | 56.6 | 5.6 | 8.87E + 05 | 11/29 | 58 | 6 ± 7 |
For each protein the molecular mass score (MOWSE), the number of peptides matched from the total number of peptides submitted to Protein Prospector, the relative molecular mass (Mr) and pI, and the overall intensity of the monoisotopic peptide peaks as a percentage of the total intensity, are given. A minimum of four peptides were required for each protein match; errors between the predicted and measured peptide masses are expressed as mean ± standard deviation (in parts per million, p.p.m).