Insets depict sample traces of force (top) and shortening (bottom) during a maximal contraction in which the fibre was switched from P0 (not shown) to three isotonic loads. For pH 7.0 and 6.2, respectively, the contractile parameters for fibre in A were Vmax = 1.62 fl s−1 and 1.38 fl s−1, P0 = 0.58 mN and 0.37 mN or 125 kN m−2 and 80 kN m−2, and the a/P0 = 0.266 and 0.304, and for the fibre in B, Vmax = 7.13 fl s−1 and 6.69 fl s−1, P0 = 0.46 mN and 0.48 mN or 169 kN m−2 and 148 kN m−2, and the a/P0 = 0.356 and 0.337.