Figure 6.

Rescue of Rab5:S34N-blocked endosome fusion by overexpression of Rabex-5. (A) Confocal fluorescence microscopy images showing the morphology of RFP-Rab5:S34N-labeled early endosomes in BHK cells with or without coexpression of GFP-Rabex-5 or GFP-Rabex-5(135-399) as indicated. Bar, 16 μm. (B) Confocal fluorescence microscopy images showing localization of GFP-Rabex-5 or GFP-Rabex-5(135-399) in the above-mentioned cells expressing RFP-Rab5:S34N. Arrows indicate partial localization of GFP-Rabex-5(135-399) on RFP-Rab5:S34N endosomes. Bar, 16 μm.