Figure 1.

DIM-7 is important for nuclear dpERK accumulation in spreading S2 cells. Integrin expressing, spreading cells were treated with control dsRNA (against the endogenous βPS) or one of two nonoverlapping dsRNAs that target DIM-7 (mskA and mskB, middle and bottom rows, respectively). The cells were fixed and stained with antibodies against DIM-7 (left column) or dpERK (right column). The DIM-7 dsRNAs reduce both DIM-7 and nuclear dpERK; mskA is slightly more effective in each assay, and was used in subsequent experiments. Bar, 10 μm.