Figure 6.

Total ERK in insulin treated, integrin expressing cells. As for spreading cells (Figure 3), total cytoplasmic ERK in control cells (expressing wild-type αPS2βPS) is found largely in aggregates (A), which tend to localize around the nucleus in cells treated with dsRNA against DIM-7 (B). Cells that express the cytoplasmic βPS-G1 mutant integrin with αPS2 (C) are more variable; most show similarities to DIM–depleted cells, although some, such as the cell on the left, look much like control cells. All cells are treated with dsRNA against the endogenous βPS gene. Bar, 10 μm.