Figure 7.

DIM-7 tyrosine phosphorylation is affected by integrins. Proteins were immunoprecipitated by anti-DIM-7 from cells 10 min after stimulation by insulin (or after no insulin, lane 1). The cell lines used were transformed to express no integrin or αPS2 in combination with the βPS subunit indicated. All cells (including controls) were grown in dsRNA to inhibit expression of endogenous βPS. Expression of the mutant βPS subunits does not significantly alter the amount of DIM-7 that is brought down, but it does reduce its tyrosine phosphorylation.