Figure 3.

The rate of flux-like motion is sensitive to kinesin-13 inhibition. (A) Prophase and early prometaphase cells were photoactivated after Kif2a/MCAK microinjection (Supplemental Videos 6 and 7). Slow P motion continues, but at a reduced rate. These cells were injected ∼6:00 (prophase) and ∼13:00 (early prometaphase) before photoactivation. Layout is as in Figure 1. (B) A single optical plane of the early prometaphase cell in A reveals buckled microtubules (arrowheads). (C) Percentage of total motion plotted against rates. Setup is identical to Figure 1. Bar, 10 μm.