Figure 4.

Monastrol treatment differentially affects slow P motion before and after NEB. (A) Prophase and early prometaphase cells were photoactivated in the presence of monastrol (Supplemental Videos 8 and 9). The frequency and rate of slow P motion is reduced pre- and post-NEB, respectively. These cells were treated with monastrol ∼124:00 (prophase) and ∼146:00 (early prometaphase) before photoactivation. Layout is as in Figure 1. (B) Maximum intensity projections through NEB of the prophase cell in A. (C) Percentage of total motion plotted against rates. Setup is identical to Figure 1. Bar, 10 μm.