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. 2007 Oct;18(10):4155–4167. doi: 10.1091/mbc.E07-02-0094

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© 2007 by The American Society for Cell Biology

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Figure 5. — Deletion within the for3p DID leads to disorganized actin cables. (A) Scheme of the Δ353-406 deletion within full-length for3p. (B) DIC image of for3Δ353-406-myc cells. (C) Projection images of spinning disk confocal stacks of AlexaFluor 488-phalloidin–stained wild-type and for3Δ(353-406)-myc cells. Note the disorganized appearance of actin cables in for3Δ(353-406)-myc cells. Yellow arrowheads indicate locations from which some actin cables seem to radiate. (D) Single focal plane widefield fluorescence images of myo52p-GFP in wild-type (left) and for3Δ(353-406)-myc (right) cells. (E) Single focal plane widefield fluorescence images of for3p-3GFP and for3pΔ(353-406)-2GFP (right). (F) Single focal plane widefield fluorescence images of for3p-3GFP (left) and for3pΔ(353-406)-2GFP (right) in cells treated with 200 μM LatA. Red arrowheads highlight cell tip localization of for3pΔ(353-406)-2GFP.