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. 2007 Oct;18(10):4155–4167. doi: 10.1091/mbc.E07-02-0094

Figure 8.

Figure 8.

Mutation of the for3p DAD domain restores wild-type actin cables and bipolar growth in bud6Δ mutants. (A) Projection images of spinning disk confocal stacks of AlexaFluor 488-phalloidin–stained for3-3GFP (wild-type), bud6Δ for3-3GFP (bud6Δ), and bud6Δ for3DAD*-2GFP (bud6Δ for3DAD*) cells. Note that the intensity of actin cables is lower in bud6Δ cells compared with wild-type cells, but that this phenotype is suppressed by the DAD* mutation. (B) Quantification of the fluorescence intensity of actin cables in for3-3GFP, for3DAD*-2GFP, bud6Δ for3-3GFP, and bud6Δ for3DAD*-2GFP cells, as in Figure 4E. (C) Calcofluor staining of bud6Δ cells expressing either for3-3GFP (wt for3) or for3DAD*-2GFP (for3DAD*), showing monopolar and bipolar growth, respectively. (D) Quantification of new end growth in strains of the indicated phenotypes. The length between the inner side of the birth scar (black line in the calcofluor staining) and the tip of the cell was measured, as shown in C. This length is of up to 1.5 μm in cells that fail to initiate growth at the new end.