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icmA disruption and Northern analysis. (A) PCR products confirming the icmA knockout. The primer sequences are described in Materials and Methods. (B) Expression profiles of icmA and car1 in wild-type (WT), icmA−, and icmA−/+ strains. RNA samples in the first lane (*) of each panel were prepared from vegetative cells that had been pulsed with 50 nM cAMP every 6 min for 5 h. Numbers correspond to hours of starvation on agar plates.
