Figure 6.

Cell mixing experiments. Wild-type and GFP-expressing icmA⁻ cells or GFP-expressing wild-type cells were mixed together at a ratio of 10:1, and then they were allowed to form fruiting bodies on nonnutrient agar plates. (A and B) GFP-expressing icmA⁻ cells stream with wild-type cells. (C) GFP-icmA⁻ cells segregate to the rear of the migrating slug. Arrow, direction of migration. (D) GFP-wild type cells are distributed throughout. (E) GFP-icmA⁻ cells deposited behind a migrating slug. (F) GFP-icmA⁻ spores (arrowheads). Single spore bodies were picked, resuspended in KK2 buffer, diluted, and plated on bacterial lawns to confirm the viability of icmA⁻ spores.