Figure 7.

Localization of and signaling by heterotrimeric G proteins. (A) Wild-type and icmA⁻ cells expressing YFP-Gγ were pulsed in shaking culture and plated on coverglasses for examination with confocal optics. (B) Cells expressing N-terminal PI3K-GFP were pulsed, plated on glass coverslips, and then stimulated with short bursts of cAMP from an adjacent micropipette. At least 12 cells were recorded in three separate experiments. (C) Quantification of the proportion of cells that redistribute N-terminal PI3K-GFP in response to cAMP. (D) Activation of ERK2 in response to cAMP treatment. (E) Quantification of maximal ERK2 response. Average of three experiments is shown. Bars, 10 μm. See Supplemental Material.