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. 2007 Oct;18(10):3752–3763. doi: 10.1091/mbc.E07-03-0281

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© 2007 by The American Society for Cell Biology

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Figure 9. — Effects of botulinum C3 exoenzyme on G2/M transition. (A) Modification of Rho GTPases by C3 exoenzyme treatment. Note the slower mobility of bands for the RhoA in the lysates of cells treated with C3 exoenzyme. (B) Effect on Ser10-phosphorylation of histone H3. Either toxin B or C3 exoenzyme or vehicle was electropermeated into HeLa cells 6.5 h after the second release. The cells were then collected at indicated times and subjected to immunoblotting for phosphoSer10-histone H3. (C) Effects on Aurora-A activation. HeLa cells treated as above were collected and subjected to immunoblotting for phosphoThr288-Aurora-A. (D) Effects on HEF1 phosphorylation. HeLa cells treated as above were collected and subjected to immunoblotting for HEF1.