Figure 4.

Activation profile of human macrophages after coculture with different human tumor cell lines. Human primary macrophages remained as controls or were incubated with MCF-7 cells, Bcl-2–overexpressing MCF-7 cells (MCF-7-Bcl-2) or RKO cells for 5 d. Residual tumor cells were removed from cocultures with 5-min accutase treatment. Afterward, 1 μg/ml LPS and 100 U IFN-γ were added to macrophages from cocultures and to control macrophages for 6 h. TNF-α, IL-10, and IL-8 contents in supernatants were quantified by FACS with BD Cytometric Bead Array Flex Sets. Data are presented as the mean ± SEM from five independent experiments. Statistically significant differences (p < 0.05) are marked with an asterisk.