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Tof1 but not Mrc1 is required for DNA replication forks to pause at chromosomal sites that correspond to tRNAs. (A) The indicated strains were released from G1 arrest at 24°C in YPD medium, and samples were taken every 15 min. DNA content was measured by flow cytometry. (B and C) Two-dimensional DNA gels were used to detect replication intermediates at sites on chromosome 6 that correspond to the tRNAs tP(UGG)F and tA(AGC)F.
