Table 2.

Extent of talinA stabilization by M7 tail fragments

	M7 levels (%)	TalinA levels (%)
Wild type	100	100
ΔF1F2	193	87
ΔMFMF	318	90
Pro1	160	10
cP	1362	81
Pro1–2	146	148
PS	59	116
SP2	160	10

Average levels of M7 and talinA protein expression in the M7 null strain expressing various M7 tail fragments. In all cases, wild-type levels are defined as 100%, and all protein measurements for the ΔF1F2, ΔMFMF and cP fragments are standardized to myoB. The Pro1–2, PS and SP2 fragments lack the M7 epitope and were detected using an anti-GFP antibody antibody. Relative levels were then determined by correlating the GFP signal to both endogenous M7 and myoB in a wild-type cell line expressing a GFP-M7 tail and comparing that to the GFP-fusions of interest. Percentages are the average of three independent experiments.