Figure 2.

Centrosome maturation is impaired in TAL-treated cells. HeLa S3 cells were transfected with Eg5 or Plk1 siRNA-oligos for 36 h or treated with 1 μM TAL for 12 h and then stained for γ-tubulin, Aurora A, or Eg5 (red; A, B, and C, respectively), pericentrin (A and B), or α-tubulin (C; green), and DNA was stained with DAPI (blue). Scale bar, 10 μm. (D) Lysates from nocodazole- (0.2 μg/mL) or taxol- (1 μg/ml) arrested cells, treated with DMSO or 1 μM TAL were used for Western blot analysis. Membranes were probed for Plk1, Eg5, γ-tubulin, Aurora A, and α-tubulin as loading control.