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. 2007 Oct;18(10):4129–4142. doi: 10.1091/mbc.E07-01-0080

Figure 3.

Figure 3.

siRNAs directed against ZW10 had little, if any, effect on minus- and plus-end–directed movement of Golgi elements or late endosomes/lysosomes; in contrast, siRNAs directed against Rab6 induced plus-end accumulation of late endosomes/lysosomes at the cell periphery. siRNA treatments were for 72 h as described in the legend to Figure 1. All images shown are full-cell confocal image projections into a single plane. (A) A′ and B′, transport (minus-end–directed transport) of tsO45G-GFP protein from ER to clustered Golgi elements in siZW10(102) occurred in the normal 30-min time frame. Cells are stained for GalT (red). (B) A′ and B′, siZW10(201) treatment for 72 h had had minor effects on the distribution of LAMP2, a late endosomal/lysosome marker. (C) Treatment of GalNAcT2-GFP HeLa cells were siRab6(554) dispersed LAMP2 to toward the cell periphery. Arrows point to LAMP2 concentrations in cell tips. (D) A′–I′, the Golgi apparatus in both siZW10(102)- and siRab6(554)-treated cells is sensitive to BFA (plus-end–directed transport) and Golgi reassembly occurred after BFA washout and transport (minus-end–directed transport) of GalNAcT2-GFP from the ER.