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. 2007 Oct;18(10):4129–4142. doi: 10.1091/mbc.E07-01-0080

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© 2007 by The American Society for Cell Biology

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Figure 6. — Rab6 depletion inhibits RINT-1 siRNA-induced dispersal of the Golgi apparatus. (A–D) Cell double-treated with Rab6 + RINT-1 siRNAs shows compact Golgi phenotype. HeLa cells stably expressing GalNAcT2-GFP were control (scrambled siRNA) (A) or RINT-1 (B), Rab6 (C), and double (Rab6 + RINT-1) (D) siRNA treated for 72 h and then fixed. Micrographs are maximum intensity projection of confocal image stacks. (E) Total cell lysates were immunoblotted with anti-RINT-1 (top row), anti-Rab6 (middle row), and anti-tubulin (bottom row) antibodies.