Figure 7.

HeLa cells double-treated with COG3 and ZW10 siRNAs display a phenotype that shows vesicular accumulation of GlcNAcT1 as observed with COG3 siRNA treatment. HeLa cells stably expressing GlcNAcT1-myc (red channel) were mock (control, A) or COG3 (B). ZW10 (C) and double (ZW10 + COG3) (D) siRNA treated for 72 h, 100 nM individual duplex siRNA concentration, fixed, and stained with anti-myc antibody, anti-GM130 (green channel); and DNA (DAPI, blue channel). Image stacks were taken with the CARV II spinning disk confocal accessory and projected into a single XY plane.