Figure 5.
17β-estradiol attenuates the self-renewal of CFU-OBs. Duplicate cultures of marrow cells in collagen gels (5 × 106 per gel) were maintained in the absence (Veh) or presence of 10–11 to 10–8 M 17β-estradiol (E2) for 6 days and then assayed for CFU-OB content. Assay of freshly isolated marrow cells indicated that there were 90 ± 14 CFU-OBs per 5 × 106 cells used to establish each culture. Thus, there was a 19.2-fold increase in CFU-OBs in cultures maintained in vehicle. The data shown represent the mean (± SEM) of CFU-OBs. AP < 0.05 treatment versus vehicle using mixed-effects ANOVA model. Linear contrast testing indicated a significant (P < 0.05) dose-dependent effect.