Figure 2.

Effect of blebbistatin on the shape, motility, cytoskeletal structure, and intracellular motion of actin and myosin II in fish epidermal keratocytes. (A) Time-lapse sequence illustrates the progressive fragmentation of the cell treated with 100 μM blebbistatin. (B) Distribution of actin and (C) distribution of microinjected rhodamine-myosin II in blebbistatin-treated keratocytes. Note the loose assemblies (arrows) of actin and myosin II at the lamellipodium–cell body transition zone (compare to distinct bundles in nontreated cells as shown in Figure 4, A and D). Velocity maps of actin in the intact cell treated with blebbistatin (D) and of actin (E) and myosin II (F) in the cell fragments generated as a result of blebbistatin treatment. Bars, 10 μm. Time is indicated in minutes:seconds. Arrows, 100 nm/s.