Figure 4.

Comparison of protein interaction maps of Ndc80p phospho-mutants. The protein interaction maps shown here summarize the results of yeast two-hybrid screens performed using wild-type Ndc80p and phospho-mutants that mimic the phosphorylation and dephosphorylation of the four N-terminal Ipl1/Aurora B consensus sites, as baits. The color of the nodes corresponds with their GO Process classification. The interactions are classified as being common to all three alleles (blue lines), exclusive of the ndc80-4A allele (green lines), or exclusive of the ndc80-4D allele (orange lines). The protein interaction maps shown here were generated with OSPREY (http://biodata.mshri.on.ca/osprey; Breitkreutz et al., 2003).