Figure 5.

In vitro proliferation of T cells derived from diabetic spleens to islet β-cell autoantigens GAD and insulin (Ins). (a) Purified T cells (2 × 10⁵/well) were cultured in medium (Click’s medium containing 5% heat-inactivated FCS) alone or with Ag’s (as indicated) in the presence of irradiated (irr.) splenocytes as Ag-presenting cells. (b) HLA restriction of the proliferative response was also tested in the assays by addition of supernatants of mAb’s to DR (HB55) and DQ (HB144). The irrelevant control Ab used in the assays was anti-IA^b (Y3JP). The cultures were pulsed with 0.5 μCi ³H after 72 hours.