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. 2007 Oct 10;2(10):e1016. doi: 10.1371/journal.pone.0001016

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Meier et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(a) Migration of NB8-E6, N91-E2, and CXCR4-overexpressing clones (NB8-CXCR4-C3, -B2, -E9, N91-CXCR4-14) towards CXCL12, in absence or presence of the CXCR4 blocker (TN14003 inhibitor). Results of a representative experiment are shown and are expressed as mean number of cells in one field. Five fields were counted for each result. The experiment was performed in triplicates and repeated three times. Error bars indicate S.D. (b) Invasion of SH-SY5Y, NB8 and N91 control and CXCR4 transduced clones through Matrigel®-coated membranes in N2 supplemented serum-free medium alone, with or without CXCL12. CXCL12-mediated invading cells (invaded cell in the absence of CXCL12 have been subtracted) -in a typical field are shown. The mean number of invading cells in 5 fields is given. Error bars indicate S.D. (* indicates p<0.05, Student's t-test)