Figure 6.

Thymic pDCs express lymphoid-associated transcripts. (a) Fresh thymic DC subsets, IL-3/CD40L–treated pDCs, and total thymocytes were analyzed for expression of mRNA for IL-3Rα, CD3γ, CD19, RAG-1, RAG-2, pre-Tα, λ-like 14.1, Spi-B, FasL, and β-actin. (b) Expression levels of Spi-B mRNA in subsets of thymic DCs. Spi-B/β-actin ratios were determined by quantitative RT-PCR. Standard curves were calculated in which the first point of the curve containing the highest amount of cDNA was set arbitrarily to 100 U for β-actin and to 560,000 U for Spi-B. Each symbol represents an independent experiment.