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ASK1-ΔNS967A does not bind either thioredoxin or 14-3-3. HA-tagged ASK1-WT, Flag-tagged ASK1-ΔN or ASK1-ΔNS967A were transiently transfected into HUVECs. Cell lysates were immunoprecipitated by Ab’s as indicated (HA, 14-3-3, thioredoxin, or Flag). ASK1-WT was detected by Western blot analysis with anti-HA (a). ASK1-ΔN and ASK1-ΔNS967A were detected by anti-Flag (b).
