Figure 1.

G protein-dependent modulation of α_1B N type whole cell currents stably expressed in HEK 293 cells (coexpressed with β_1b + α₂δ subunits). Inclusion of 5 nM purified G_βγ in the patch pipette in the presence and the absence of a strong depolarizing prepulse (+150 mV for 50 ms). The cell was bathed in 20 mM barium, currents were elicited by stepping from a holding potential of −100 mV to a test potential of +5 mV with or without application of a strong depolarizing prepulse (+150 mV for 50 ms, see Inset). The records were leak- and capacitance-subtracted by using a standard p/5 protocol.