Figure 1.

Experimental design. Transgenic males lacking sperm and Acps, and mutant males lacking sperm were used to assess the role of these seminal fluid components in regulating female attractiveness. Virgin females were mated to DTA males (transfer neither Acps nor sperm), spermless males (transfer Acps only), or control males (transfer both). At 8, 10, or 24 h after the end of mating, the attractiveness of these mated females and that of age-matched virgin females were measured.