Expression of UCP1, UCP2, and UCP3 in IBAT of B6 and A/J mice. After 2 weeks of either a low fat or a high fat diet, total cellular RNA was prepared from IBAT and examined for the expression of UCP1, UCP2, UCP3, and cyclophilin by Northern blot hybridization. (A) Representative Northern blots. (B–D) Average of results for each UCP from six mice of each strain. ∗, UCP1 levels in high fat fed mice were greater than in low fat mice (B6: P < 0.03; A/J: P < 0.0001). #, UCP1 levels in A/J H mice were greater than in B6 H mice (P < 0.005).