Table 2.
Specificities and compositions of glycosidase mixtures
| Enzyme | Source | Linkage specificity | Concentration (units/ml) | pH optimum | Exoglycosidase digests (number of 0.5 μl aliquots)
|
|||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| A | B | C | D | E | F | G | H | |||||
| Sialidase | Arthrobacter ureafaciens | α2–6 > α3, α8 | 1 | 5.0–5.5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Galactosidase | Streptococcus pneumoniae | β1–4 | 0.2 | 5.5–6.5 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |
| GlcNAcase | Chicken liver | β1–2, α3, α4, α6 | 1 | 5.0–5.5 | 1 | 1 | 1 | 1 | ||||
| GlcNAcase | Streptococcus pneumoniae | β1–2 | 0.008 | 4.0–4.5 | 1 | 1 | ||||||
| Mannosidase | Jack bean | α1–3 | 0.1 | 4.0–4.5 | 1 | |||||||
| Mannosidase | Jack bean | α1–2, α3, α6 | 5 | 4.0–4.5 | 1 | 1 | 1 | |||||
| Mannosidase | Helix pomatia | β1–4 | 1 | 4.0–4.5 | 1 | |||||||
| Sample buffer | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | ||||
| 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | |||||
| 10% methanol* | 7 | 6 | 5 | 4 | 4 | 3 | 5 | 4 | ||||
Glycosidase mixtures are adapted from ref. 2.
*
Sample buffer includes 50 mM sodium citrate/phosphate (pH 5.0), 25 mM zinc chloride.